In this week-long hands-on workshop, attendees will be immersed in a High Performance Computing environment and will learn the current approaches to rapidly go from electron micrographs all the way to creating a high quality PDB molecular interpretation of the results.

The entire course will be in the context of a single real-world dataset on a clinically relevant G protein-coupled  receptor and will cover the essence of the theoretical components of image signal analysis as well as provide genuine hands-on experience for the attendees.

The material covered will be:

• Setting up consistent cryoEM data structures
• Motion correction
• CTF estimation
• AI techniques for particle picking
• ab initio 3D model generation
• Particle homogenization (2D and 3D classification techniques)
• 3D variability analysis and how it can inform further 3D refinement strategies
• High resolution 3D refinement strategies
• Advanced 3D masking
• Molecular Dynamics Flexible Fitting (MDFF) for PDB model fitting
• Realspace refinement strategies for PDB finalization
• PDB validation

The softwares covered will include the most up to date versions of:

• RELION
• CRYOSPARC
• EMAN
• ChimeraX / iSOLDE
• Phenix
• Coot

All attendees will have priority access to a high-performance computing system during the course and will only be required to bring their own laptop to access it.

The course will be fully catered for with morning tea, lunch and afternoon tea provided each day. The price does not include accommodation, but the Monash Institute of Pharmaceutical Sciences is centrally situated in the city of Melbourne and we will provide suggestions of places to stay that are close either by public transport or within walking distance.

Venue:
Monash Institute of Pharmaceutical Sciences
399 Royal Pde, PARKVILLE
Date: Monday July 7 to Friday July 11, 2025
Time: 9:00 AM - 5:00 PM

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